| 目录: 11070 |
| 产品名称: Human NPC1L1 Full Length Protein |
| 规格: 10 µg, 50 µg and 100 µg |
| 基因符号: LDLCQ7; NPC11L1; SLC65A2 |
| Target: NPC1L1 |
| UNIPROT ID: Q9UHC9 |
| 描述: Human NPC1L1 full length protein-synthetic nanodisc |
| 背景: The protein is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. |
| Species/Host: HEK293 |
| Molecular Weight: The human full length NPC1L1 protein has a MW of 148.7 kDa |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure1. Elisa plates were pre-coated with Flag Tag NPC1L1-Nanodisc (0.2 µg/per well). Serial diluted Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for Flag monoclonal antibody binding with NPC1L1-Nanodisc is 4.526ng/ml. | 
Figure2. Human NPC1L1-Nanodisc, Flag Tag on SDS-PAGE |
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