Arf1(Δ17Q71L) Protein

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Arf1 Protein Δ17 Q71L mutant

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Cat.#: 10123

Size: 10 μl

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Arf1(Δ17Q71L) Mutant

货号: 10123

产品名称: Arf1 Protein Δ17 Q71L mutant

基因符号: ADP-ribosylation factor 1

Source: Human recombinant, His6-tag

Expression 种属反应性: E. coli

Molecular Weight: 21 kDa

纯化:: >95% by SDS-PAGE

Introduction: Arf1 is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport.

Amino Acid Sequence (1-181, Δ17, Q71L)

MGNIFANLFKGLFGKK-MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWD
VGGLDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAM
NAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Properties

Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.

Physical Appearance (form): White or clear

浓度: 1 mg/mL

Storage: -80°C

Preparation Instructions:
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside(DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Arf1 Δ17Q71L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining

References:
1. Amor, J. C. et al., Nature 372: 704-708, 1994.
2. Bobak, D. A. et al., Proc. Nat. Acad. Sci. 86: 6101-6105, 1989.
3. Hirai, M. et al., Genomics 34: 263-265, 1996.
4. Kumari, S. et al., Cell Biol. 10: 30-41, 2008.
5. Lee, C.-M. et al., J. Biol. Chem. 267: 9028-9034, 1992.
6. Mossessova, E. et al., Cell 92: 415-423, 1998.
7. Peng, Z. G. et al., Biofactors 2: 45-49, 1989.
8. Presley, J. F. et al., Nature 417: 187-193, 2002.
9. Renault, L. et al., Nature 426: 525-530, 2003.

Arf6	Arl1	Arl3	Cdc42	Gα₁₂	Gα₁₃	Gα₁₄	Gα_i	Gα_o	Gα_q
Gα_s	Gα_z	Rab11	Rab12	Rab14	Rab17	Rab18	Rab25	Rab26	Rab28
Rab2A	Rab3	Rab30	Rab31	Rab35	Rab38	Rab39	Rab41	Rab4A	Rab5
Rab7	Rab8A	Rab9A	Ral	Rac1	Ran	Rap1	Ras	Rheb	RhoA	Sar1

Albumin	Apolipoprotein	BAG1	BMP3	Cystatin	EYA2
FASCIN	GAPDH	GRB7	KIM1	MUC1	ORAIL
RAC-8A	SEPTIN9	SLC7A5	SUMO	Survivin	TFF3
Vimentin	Transferrin	β-Actin	β-Tubulin

ABL	AKT	ALK	ATM	ß-CATENIN	BRAF	BRCA1
BRCA2	CTCF	CTNNB1	EGFR	ERBB2	FBXW7	FGFR1
GNAQ	HRAS	IDH	JAK2	KIT	MEK1	NFE2L2
NOTCH1	PIK3CA	PTEN	RAS	RET	RHEB	SMAD4
SMO	STK11	TP53

Rab5 -GTP IF, IHC and IP	Rap1-GTP IF, IHC and IP
Ral-GTP IF, IHC and IP	Ran-GTP IF, IHC and IP
Sar1-GTP IF, IHC and IP	Arf6-GTP IF, IHC and IP
Rab11-GTP IF, IHC and IP

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