关于 NewEast Biosciences
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。

¥4,800.00
| 货号: 26444 |
| 规格: 100 µL |
| 基因符号: NOTCH1, AOS5, AOVD1,TAN1, hN1 |
| 描述: NOTCH1(M2011R) 小鼠单克隆抗体 |
| 背景: NOTCH1 is a human gene encoding a single-pass transmembrane receptor. It is a member of the NOTCH family. NOTCH family members play a role in a variety of developmental processes by controlling cell fate decisions. Diseases associated with NOTCH1 include aortic valve disease 1 and adams-oliver syndrome 5, and so on. An important paralog of this gene is NOTCH2. |
| 免疫原: A synthetic peptide from the internal region of NOTCH1 which includes the mutation of M2011R, human origin. |
| 经过测试的应用: ELISA, WB, IF, IHC |
| 推荐稀释度: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
| 浓度: 1 mg/ml |
| 种属反应性: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯化:: Purified from ascites |
| Preservative: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes M2011R mutant, but not wild type NOTCH1 of vertebrates. |
| 储存条件:: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant NOTCH1(M2011R) and wild type proteins. Purified His-tagged NOTCH1(M2011R) protein (lane1) and corresponding wild type protein (lane2) were blotted with NOTCH1(M2011R) monoclonal antibody (货号26444). |
Immunofluorescence:
Immunofluorescence of cells expressing NOTCH1 proteins with NOTCH1(M2011R) antibody. HEK293T cells were transfected with pCDNA3-GFP-NOTCH1 (WT) plasmid (left column) or pCDNA3-GFP-NOTCH1(M2011R) plasmid (right column), then fixed and stained with NOTCH1(M2011R) monoclonal antibody (货号26444). |