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Human PD-1 Protein, His tag

Recombinant human PD-1 protein with C-terminal 6XHis tag
5/5

¥3,400.00

Cat.#:  11273

   Size:   100 μg

In Stock

          Product Description          
目录:     11273
产品名称:   Human PD-1 Protein
规格:    10 µg, 50 µg and 100 µg
基因符号:   PDCD1;PD1;CD279;SLEB2
Target:    PD-1
UNIPROT ID:    Q15116
描述:    Recombinant human PD-1 protein with C-terminal 6xHis tag
背景:     This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 16.8 kDa after removal of the signal peptide.
Molecular Characterization:    PD-1(Leu25-Gln167) 6×His tag
纯化::    The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human PD-1 Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well)Human PD-1 Protein,His tag (11273) can bind PD-1 antibody(DM177), Rabbit mAb in a linear range of 0.06-1.60 ng/mL.
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