| 货号: 11683 |
| 产品全名: 人 EGF 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 Urogastrone |
| 目标蛋白: EGF |
| UNIPROT ID: P01133 |
| 描述: Recombinant human EGF protein with N-terminal human Fc tag |
| 背景: This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016] |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 32.4 kDa after removal of the signal peptide. The apparent molecular mass of hFc-EGF is approximately 35-45 kDa due to glycosylation. |
| 分子特征: hFc(Glu99-Ala330) EGF( Asn971- Arg1023) |
| 纯化: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human EGF Protein, hFc Tag on SDS-PAGE under reducing condition. |
