| 货号: 11289 |
| 产品全名: 人 CTLA-4 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 CTLA4;CD152 |
| 目标蛋白: CTLA-4 |
| UNIPROT ID: P16410 |
| 描述: Recombinant human CTLA-4 protein with C-terminal human Fc tag |
| 背景: This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 39.6 kDa after removal of the signal peptide.The apparent molecular mass of CTLA4-hFc is approximately 40-55 kDa due to glycosylation. |
| 分子特征: CTLA-4(Gln36-Ser161) hFc(Glu99-Ala330) |
| 纯化: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human CTLA-4 Protein, hFc Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CTLA-4, hFc tagged protein (11289) can bind Anti-CTLA4 Neutralizing antibody 28031 in a linear range of 0.64-80.0 ng/ml. | 
Figure 3. HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CTLA4, hFc tagged protein (11289) 1µg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody. | 
Figure 4. Flow cytometry analysis with 1ug/ml Human CTLA4 Protein, hFc tag (11289) on Expi293 cells transfected with human B7-1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram). |
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