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Human CD47 Full Length Protein

Human CD47 Full Length Protein-Synthetic Nanodisc
5/5

¥38,200.00

Cat.#:  11020

   Size:   50 μg

3-4 weeks

          Product Description          
目录:     11020
产品名称:   Human CD47 Full Length Protein
规格:    10 µg, 50 µg and 100 µg
基因符号:   IAP; MER6; OA3
Target:    CD47
UNIPROT ID:    Q08722
描述:    Human CD47 full length protein-synthetic nanodisc
背景:     A membrane protein involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes.
Species/Host:    HEK293
Molecular Weight:    The human full length CD47 protein has a MW of 35.2 kDa
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag CD47-Nanodisc (0.2 µg/per well). Serial diluted CD47 monoclonal antibody (28058) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for CD47 monoclonal antibody binding with CD47-Nanodisc is 2.959ng/ml.

Figure2. Human CD47-Nanodisc, Flag Tag on SDS-PAGE
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