| 货号: 11232 |
| 产品全名: 人 TPBG 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 5T4;5T4AG;M6P1;WAIF1 |
| 目标蛋白: 5T4 |
| UNIPROT ID: Q13641 |
| 描述: Recombinant Human 5T4 Protein with C-terminal 6xHis tag |
| 背景: This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 36.1 kDa after removal of the signal peptide.The apparent molecular mass of 5T4-His is approximately 55-70 kDa due to glycosylation. |
| 分子特征: 5T4(Ser32-Ser355) 6×His tag |
| 纯化: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human 5T4 Protein, His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human 5T4 Protein, His Tag (11232) can bind Anti-5T4 Rabbit mAb in a linear range of 3.2-16 ng/mL. | 
Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human 5T4 Protein, His Tag (11232) can bind Anti-5T4 (H8 biosimilar) mAb (28149) in a linear range of 0.64-16 ng/mL. | 
Figure 4. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human 5T4 Protein, His Tag (11232) can bind Anti-5T4 (GEN-1044 biosimilar) mAb (28150) in a linear range of 0.64-16 ng/mL. |
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