| 货号: 11231 |
| 产品全名: 人 PMEL 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 D12S53E;gp100;ME20;ME20-M;ME20M;P1;P100;PMEL17;SI;SIL;SILV |
| 目标蛋白: PMEL |
| UNIPROT ID: P40967 |
| 描述: Recombinant human PMEL Protein with C-terminal Human Fc tag |
| 背景: This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011] |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 46.2 kDa after removal of the signal peptide.The apparent molecular mass of PMEL-hFc is approximately 130-250 kDa due to glycosylation. |
| 分子特征: PMEL(Lys25-Ala595) hFc(Glu99-Ala330) |
| 纯化: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human PMEL Protein, hFc Tag on SDS-PAGE under reducing condition. |
