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Human TRPA1 Full Length Protein

Human TRPA1 Full Length Protein-Synthetic Nanodisc
5/5

¥30,000.00

货号:11016

规格: 50 μg

库存状态:In Stock

           产品描述          
货号:   11016
产品全名:   人 TRPA1 Full Length 蛋白
规格:   10/50/100 µg
基因符号   ANKTM1; FEPS; FEPS1
目标蛋白:    TRPA1
UNIPROT ID:    O75762
描述:   人 TRPA1 full length protein-synthetic nanodisc
背景:     The structure of the protein is highly related to both the protein ankyrin and transmembrane proteins. This protein is activated by a large variety of structurally unrelated electrophilic and non-electrophilic chemical compounds. Electrophilic ligands activate TRPA1 by interacting with critical N-terminal Cys residues in a covalent manner, whereas mechanisms of non-electrophilic ligands are not well determined. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.
物种/宿主:    HEK293
分子量:   The human full length TRPA1 protein has a MW of 127.5 kDa
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag TRPA1-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with TRPA1-Nanodisc is 7.433ng/ml.

Figure2. Human TRPA1-Nanodisc, Flag Tag on SDS-PAGE
           引用文献