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SMAD4(D351H)

Anti-SMAD4(D351H) Mouse Monoclonal Antibody
5/5

¥4,800.00

货号:26418

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   SMAD4(D351H) 小鼠单抗
货号:   26418
基因符号:   SMAD4, DPC4, JIP, MADH4, MYHRS
描述:   SMAD4(D351H) 小鼠单抗
背景:   SMAD4 family member 4, also known as SMAD4, is a protein that in human is encoded by the SMAD4 gene. It is often found mutated in many cancers. It acts as a tumor suppressor that functions in the regulation of the TGF-? signal transduction pathway, which negatively regulates growth of epithelial cells and the extracellular matrix (ECM). SMAD4 alterations have been found in multiple colorectal cancer and pancreatic carcinoma. It is found inactivated in at least 50% of pancreatic cancers. It is also found mutated in the autosomal dominant disease juvenile polyposis syndrome (JPS).
免疫原:   A synthetic peptide from the internal region of SMAD4 which includes the mutation of D351H, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   ELISA: 1:1000-1:2000WB: 1:500-1:1000IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl and 50% glycerol
种属反应性:  Recognizes D351H mutant, but not wild type SMAD4 of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:  Western blot analysis of recombinant SMAD4(R361H) and wild type proteins. Purified His-tagged SMAD4(R361H) protein (lane2) and corresponding wild type protein (lane1) were blotted with Anti-SMAD4(R361H) monoclonal antibody (货号:26422).Immunofluorescence:  Immunofluorescence of cells expressing SMAD4 proteins with Anti-SMAD4(R361H) antibody. HEK293T cells were transfected with pCDNA3-GFP-SMAD4(R361H) plasmid pCDNA3-GFP-SMAD4 (WT) plasmid, pCDNA3-GFP-SMAD4 (R361S) plasmid or pCDNA3-GFP-SMAD4 (R361C) plasmid, then fixed and stained with Anti-SMAD4(R361H) monoclonal antibody (货号:26422).
           引用文献