| 货号: 11164 |
| 产品全名: SARS-CoV-2 (2019-NCoV) S Protein RBD |
| 规格: 10/50/100 µg |
| 基因符号 S protein RBD;Spike glycoprotein Receptor-binding domain;S glycoprotein RBD;Spike protein RBD;COVID-19 |
| 目标蛋白: S protein RBD |
| UNIPROT ID: P0DTC2 |
| 描述: Recombinant SARS-CoV-2 (2019-nCoV) S protein RBD with C-terminal 6xHis tag |
| 背景: SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response. |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 37-38 kDa after removal of the signal peptide. |
| 分子特征: S protein RBD(Arg319-Phe541) 6×His tag |
| 纯化: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. SARS-CoV-2 (2019-nCoV) S protein RBD, His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) SARS-CoV-2 (2019-nCoV) S protein RBD, His tagged protein (11164) can bind its native ligand Human ACE2, hFc tagged protein 11196 in a linear range of 1.96-31.72 ng/ml. |
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