| 货号: 28493 |
| 产品全名: ROR1(DM149) 兔单克隆抗体 |
| 基因符号 ROR1;NTRKR1 |
| 描述: ROR1 antibody(DM149) 兔单克隆抗体 |
| 背景: This gene encodes a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with B-cell chronic lymphocytic leukaemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq; Jun 2012] |
| 经过测试的应用: ELISA; Flow Cyt |
| 推荐稀释比: ELISA 1:5000-10000; Flow Cyt 1:100 |
| 种属反应性: Rabbit |
| 亚型: Rabbit IgG |
| 纯化: Purified from cell culture supernatant by affinity chromatography |
| 种属反应性: 人 ROR1 |
| 成分: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
Figure 1. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human ROR1 protein, His tagged protein 11267 can bind Rabbit anti-ROR1 monoclonal antibody (clone: DM149) in a linear range of 1-50 ng/ml. | 
Figure 2. ROR1 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-ROR1 (DM149) on Expi293 cells transfected with human ROR1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram). |
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