武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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CTLA4 mAb (Reference mAb)

Anti-CTLA4 Monoclonal Antibody (Reference? Monoclonal Antibody)?
5/5

¥199.00

货号:28031

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   28031
产品全名:   CTLA4 Reference 单抗
基因符号   CTLA4;CD152
描述:   CTLA4 Reference 单抗
背景:   Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
经过测试的应用:   ELISA; Flow Cyt
推荐稀释比:   ELISA 1:5000-10000; Flow Cyt 1:100
种属反应性:   Homo sapiens
亚型:   IgG1
纯化:   Purified from cell culture supernatant by affinity chromatography
种属反应性:   人 CTLA-4
成分:   Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CTLA4, mFc-His tagged protein 11145 can bind Anti-CTLA4 Neutralizing antibody in a linear range of 0.13-16.0 ng/ml.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CTLA4, hFc tagged protein (11289) can bind Anti-CTLA4 Neutralizing antibody in a linear range of 0.64-80.0 ng/ml. In order to specifically detect 28031, mouse anti-human Fab-specific antibody was used as detection antibody.

Figure 3. HEK293 cell line transfected with irrelevant protein  (B) and human CTLA4  (A) were surface stained with anti-CTLA4 neutralizing antibody 1µg/ml (ipilimumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.
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