关于 NewEast Biosciences
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。

¥4,800.00
| 货号: 26038 |
| 基因符号: Ras(G13D) |
| 描述: Ras(G13D) 小鼠单克隆抗体 |
| 背景: Ras(G13D) is one type of Ras missense mutations at the codon 13. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G13D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers. |
| 免疫原: A synthetic peptide from the internal region of Ras which includes the mutation of G13D, human origin. |
| 经过测试的应用: ELISA, WB, IF, IHC |
| 推荐稀释度: ELISA: 1:100-1:3000 WB: 1:200-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
| 浓度: 1 mg/ml |
| 种属反应性: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG2b |
| 纯化:: Purified from ascites |
| Preservative: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl and 50% glycerol |
| 种属反应性: Ras(G13D) monoclonal antibody recognizes RasG13D proteins of vertebrates. |
| 储存条件:: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant Ras WT and G13D proteins with Ras(G13D) monoclonal antibody. Equal amounts of purified Ras WT (lane 1, Cat # 10172) and G13D (lane 2, Cat # 10155) proteins were loaded and blotted with Ras(G13D) mouse monoclonal antibody (Cat # 26038) (top panel). Input control (bottom panel) was blotted with rabbit polyclonal Ras antibody (Cat # 21021).
Immunofluorescence:
Immunofluorescence of cells expressing RAS proteins with Ras(G13D) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(G13D) plasmid,pCDNA3-GFP-Ras(WT)plasmid, pCDNA3-GFP-Ras(G13A) plasmid,pCDNA3-GFP-Ras(G13V) plasmid, pCDNA3-GFP-Ras(G13C)plasmid, pCDNA3-GFP-Ras(G12R)plasmid or pCDNA3-GFP-Ras(G13S)plasmid, then fixed and stained with Ras(G13D) monoclonal antibody (货号 26038). |