关于 NewEast Biosciences
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。

¥4,800.00
| Rab4GTP Monoclonal Antibody |
| 货号: 26921 |
| 规格: 30 µL |
| 基因符号: RAB4 |
| 描述: Rab4-GTP Monoclonal Antibody |
| 背景: Rab-4 belongs to the small GTPase Rab family. It interacts with RAB11FIP1, RABEP1, ZFYVE20, RUFY1, SGSM1, SGSM2 and SGSM3. It participates in protein transport and in vesicular traffic. |
| 免疫原: Recombinant full length protein of active Rab4A. |
| 经过测试的应用: IP, IHC and IF (Not applicable for WB since SDS denatures Rab4 GTPase) |
| 推荐稀释度: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| 浓度: 1 mg/ml |
| 种属反应性: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯化:: Purified from ascites |
| Preservative: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Rab4-GTP monoclonal antibody recognizes active Rab4 from vertebrates. |
| 储存条件:: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation/Western blot:
Rab4A Activation Assay.
Purified GST-tagged Rab4A proteins (货号10130) were immunoprecipitated with the Rab4-GTP monoclonal antibody (Cat. No. 26921) after treated with GDP (lane 1) or GTPγ S (lane 2), and was blotted with Rab4A polyclonal antibody (Cat. No. 21067). Input control is shown in the bottom panel. |