关于 NewEast Biosciences
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。

¥4,800.00
| Rab35GTP Monoclonal Antibody |
| 货号: 26922 |
| 规格: 30 µL |
| 基因符号: RAB35 |
| 描述: Rab35-GTP Monoclonal Antibody |
| 背景: The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions. |
| 免疫原: Recombinant full length protein of active Rab35 |
| 经过测试的应用: IP, IHC and IF (Not applicable for WB since SDS denatures Rab35 GTPase) |
| 推荐稀释度: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| 浓度: 1 mg/ml |
| 种属反应性: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯化:: Purified from ascites |
| Preservative: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Rab35-GTP monoclonal antibody recognizes active Rab35 proteins of vertebrates. |
| 储存条件:: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation/Western blot:
Rab35 Activation Assay.
Purified full-length Rab35 proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the Rab35-GTP monoclonal antibody (Cat. No. 26922). Immunoblot was with an Rab35 polyclonal antibody (Cat. No. 21078). |
| 1. IL-17A Recruits Rab35 to IL-17R to Mediate PKCα-Dependent Stress Fiber Formation and Airway Smooth Muscle Contractility J Immunol. 2019 2019 PMID: 30683702 |
| 2. Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway J Neurosci. 2016 PMID: 27535913 |
| 3. Molecular Mechanisms of Synaptic Vesicle Degradation academiccommons.columbia. 2016 PMID: |
| 4. Calcium dependent regulation of Rab activation and vesicle fusion by an intracellular P2X ion channel Nat Cell Biol. 2014 PMID: 24335649 |