关于 NewEast Biosciences
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。

¥4,800.00
| Rab3GTP 小鼠单克隆抗体 |
| 货号: 26920 |
| 规格: 30 µL |
| 基因符号: RAB3 |
| 描述: Rab3-GTP 小鼠单克隆抗体 |
| 背景: Rab3 is a member of the Rab-family GTPases and is specifically targeted to synaptic vesicles. |
| 免疫原: Recombinant full length protein of active Rab3A |
| 经过测试的应用: IP, IHC and IF (Not applicable for WB since SDS denatures Rab3 GTPase) |
| 推荐稀释度: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| 浓度: 1 mg/ml |
| 种属反应性: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯化:: Purified from ascites |
| Preservative: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Rab3-GTP monoclonal antibody recognizes active Rab3 proteins of vertebrates. |
| 储存条件:: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation/Western blot:
Rab3 activation assay.
Purified full-length Rab3A proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the Rab3-GTP monoclonal antibody (Cat. No. 26920). Immunoblot was with an Rab3A polyclonal antibody (Cat. No. 21041). |
| 1. A tumor-specific modulation of heterogeneous ribonucleoprotein A0 promotes excessive mitosis and growth in colorectal cancer cells Cell Death Dis. 2020 PMID: 32303675 |
| 2. Manganese-induced alpha-synuclein overexpression impairs synaptic vesicle fusion by disrupting the Rab3 cycle in primary cultured neurons Toxicol Lett. 2018 PMID: 29289693/ |
| 3. Mutant Huntingtin Impairs BDNF Release from Astrocytes by Disrupting Conversion of Rab3a-GTP into Rab3a-GDP J Neurosci. 2016 PMID: 27559163 |