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Rab11 Protein

Rab11 Protein
5/5

¥347.00

货号:10126

规格: 10 微升

库存状态:In Stock

           产品描述          
产品名称:   Rab11 蛋白
货号   10126
产品全名:   Rab11 蛋白
基因符号  Rab-protein 11, CG5771, DmRab11, Drab11, Rab-r11, RAB11
Source:   Human, recombinant full length, His6-tag
Expression 种属反应性:    E. coli
分子量:   18 kDa
纯化:   >95% by SDS-PAGE
Introduction:   Rab11 is a small GTP-binding proteins of the Rab family, such as Rab11A, play essential roles in vesicle and granule targeting. Rab11 is also a GTPase that regulates endosomal trafficking to apical plasma membrane domains in polarized epithelial cells.
Amino Acid Sequence  (1-173)
MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDT
AGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAV
PTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIY
Properties
Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form):   White or clear
Concentration:   1 mg/mL
Storage:    -80°C
Preparation Instructions:   
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Rab11 was determined by SDS- PAGE and Coomassie Brilliant Blue Staining.


References:   
1. Bao, X. et al., Europ. J. Biochem. 269: 259-271, 2002.
2. Gromov, P. S. et al., FEBS Lett. 429: 359-364, 1998.
3. Casanova, J. E. et al., Molec. Biol. Cell 10: 47-61, 1999.
4. Drivas, G. T. et al., Oncogene 6: 3-9, 1991.
5. Gromov, P. S. et al., FEBS Lett. 429: 359-364, 1998.
6. Guichard, A. et al., Nature 467: 854-858, 2010.
7. Larance, M. et al., J. Biol. Chem. 280: 37803-37813, 2005.
8. Shirane, M. et al., Science 314: 818-821, 2006.

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