| 货号: 12174 |
| 产品全名: Mouse SIRPa 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 SHP substrate 1;SHPS-1;Bit;MyD-1 antigen;mSIRP-alpha1;p84;CD172a |
| 目标蛋白: SIRPa |
| UNIPROT ID: P97797 |
| 描述: Recombinant mouse SIRPa protein with C-terminal 6xHis tag |
| 背景: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (By similarity).[UniProtKB/Swiss-Prot Function] |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 38.7kDa after removal of the signal peptide. The apparent molecular mass of mSIRPa-His is approximately 55-70 kDa due to glycosylation. |
| 分子特征: Mouse SIRPa(Lys32-Asn373) 6×His tag |
| 纯化: The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Mouse SIRPA Protein, His Tag on SDS-PAGE under reducing condition. |
