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IDH2(R140Q)

Anti-IDH2(R140Q) Mouse Monoclonal Antibody
5/5

¥4,800.00

货号：26165

规格： 100 微升

库存状态：In Stock

           产品描述          
产品名称:   IDH2(R140Q) 小鼠单抗
货号:   26165
基因符号:   IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH
描述:   IDH2(R140Q) 小鼠单抗
背景:   Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
免疫原 :   A synthetic peptide from the internal region of IDH2 which includes the mutation of R140Q, human origin.
经过测试的应用:   ELISA, WB, IF, IHC
推荐稀释比:   
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IF: 1:50-1:100
IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性：  Monoclonal
亚型:   
纯化:   Purified from ascites
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes R140Q mutant, but not wild type IDH2 of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing

蛋白质印迹:   

Western blot analysis of recombinant IDH2(R140Q) and wild type proteins.

Purified His-tagged IDH2(R140Q) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with anti IDH2(R140Q) monoclonal antibody (货号:26165).

Immunofluorescence:   

Immunofluorescence of cells expressing IDH2 proteins with anti IDH2(R140Q) antibody.

HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R140Q) plasmid (right column), then fixed and stained with anti-IDH2(R140Q) monoclonal antibody (货号:26165).

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           引用文献          
01. IDH2 R172 Mutations Across Poorly Differentiated Sinonasal Tract Malignancies : Forty Molecularly Homogenous and Histologically Variable Cases With Favorable Outcome
Am J Surg Pathol. 2021
02. Immunohistochemical analysis of IDH2 R172 hotspot mutations in breast papillary neoplasms: applications in the diagnosis of tall cell carcinoma with reverse polarity
Modern Pathology, 2020
03. Molecular epidemiology of IDH2 hotspot mutations in cancer and immunohistochemical detection of R172K, R172G, and R172M variants
Hum Pathol., 2020
04. The role of a monoclonal antibody 11C8B1 as a diagnostic marker of IDH2-mutated sinonasal undifferentiated carcinoma
Mod Pathol., 2019
05. DNA methylation-based classification of sinonasal undifferentiated carcinoma
Modern Pathology, 2019
06. Vulnerabilities in mIDH2 AML confer sensitivity to APL-like targeted combination therapy
Cell Res., 2019
07. Multiple Ways to Detect IDH2 Mutations in Angioimmunoblastic T-Cell Lymphoma from Immunohistochemistry to Next-Generation Sequencing
J Mol Diagn., 2018
08. On the Utility of Short Echo Time (TE) Single Voxel 1H–MRS in Non–Invasive Detection of 2–Hydroxyglutarate (2HG); Challenges and Potential Improvement Illustrated with Animal Models Using MRUI and LCMode
PLoS One. 2016
09. Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
Brain Tumor Pathol. 2015
10. Mutant IDH inhibits HNF-4a to block hepatocyte differentiation and promote biliary cancer
Nature, 2014
11. IDH2 Mutation Analysis in Undifferentiated and Poorly Differentiated Sinonasal Carcinomas for Diagnosis and Clinical Management
Am J Surg Pathol. 2020
12. Metabolomic comparison between cells over-expressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
J Neurochem. 2015
13. Vulnerabilities in mIDH2 AML confer sensitivity to APL-like targeted combination therapy
Cell Res. 2019
14. Multiple Ways to Detect IDH2 Mutations in Angioimmunoblastic T-Cell Lymphoma from Immunohistochemistry to Next-Generation Sequencing
J Mol Diagn. 2018
15. The IDH2 R172K mutation associated with angioimmunoblastic T-cell lymphoma produces 2HG in T cells and impacts lymphoid development
Proc Natl Acad Sci U S A. 2016
16. The pathological features of angioimmunoblastic T-cell lymphomas with IDH2 R172 mutations
Mod Pathol. 2019
17. Expression of TFH Markers and Detection of RHOA p.G17V and IDH2 p.R172K/S Mutations in Cutaneous Localizations of Angioimmunoblastic T-Cell Lymphomas
Am J Surg Pathol. 2017
18. Molecular Pathology of Breast Tumors: Diagnostic and Actionable Genetic Alterations
Surg Pathol Clin. 2021
19. Angioimmunoblastic T-Cell Lymphoma and Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma: A Novel Form of Composite Lymphoma Potentially Mimicking Richter Syndrome
Am J Surg Pathol. 2021
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