| 货号: 11222 |
| 产品全名: 人 VEGFR2 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 CD309;FLK1;VEGFR;VEGFR2 |
| 目标蛋白: VEGFR2 |
| UNIPROT ID: P35968 |
| 描述: Recombinant human VEGFR2 Protein with C-terminal 6xHis tag |
| 背景: Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009] |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 84.1kDa after removal of the signal peptide.The apparent molecular mass of VEGFR2-His is approximately 130-250 kDa due to glycosylation. |
| 分子特征: VEGFR2(Ala20-Glu764) 6×His tag |
| 纯化: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human VEGFR2 Protein, His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 5 µg/ml (100 µl/well) Human VEGFR2 protein, His Tag (11222) can bind Anti-VEGFR2 Neutralizing antibody 28067 in a linear range of 6.4-800 ng/mL. |
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