| 货号: 11199 |
| 产品全名: 人 TNFa 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 DIF;NF-alpha;NFA;NFSF2;achexin;achectin;NFa |
| 目标蛋白: TNFA |
| UNIPROT ID: P01375 |
| 描述: Recombinant human TNFa protein with C-terminal 6xHis tag |
| 背景: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 18.1 kDa after removal of the signal peptide. |
| 分子特征: TNFa(Val77-Leu233) 6×His tag |
| 纯化: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human TNFα Protein, His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human TNFa , His tagged protein (11199) can bind Anti-TNFa Neutralizing antibody 28064 in a linear range of 0.64-16 ng/ml. |
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