| 货号: 11217 |
| 产品全名: 人 IL2RA 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 IL2RA;D25;55;L2-RA;L-2-RA |
| 目标蛋白: IL2RA |
| UNIPROT ID: P01589 |
| 描述: Recombinant human IL2RA Protein with C-terminal 6xHis tag |
| 背景: The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 22.6 kDa after removal of the signal peptide.The apparent molecular mass of IL2RA-His is approximately 35-55 kDa due to glycosylation. |
| 分子特征: IL2RA(Glu22-Cys213) 6×His tag |
| 纯化: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human IL2RA Protein, His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human IL2RA, His tagged protein (11217) can bind Anti-IL2RA Neutralizing antibody 28052 in a linear range of 0.64-400 ng/ml. | 
Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human IL2RA Protein, His Tag (11217) can bind Human IL2 Protein, hFc Tag 11256 in a linear range of 0.64-400 ng/mL. | |
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