| 货号: 12088 |
| 产品全名: 人 IL-4 (C-Fc) 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 Interleukin-4;IL-4;B-Cell Stimulatory Factor 1;BSF-1;Binetrakin;Lymphocyte Stimulatory Factor 1;Pitrakinra;IL4 |
| 目标蛋白: IL-4 |
| UNIPROT ID: P05112 |
| 描述: Recombinant Human Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His25-Ser153 is expressed with a Fc tag at the C-terminus. |
| 背景: Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4 T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response. |
| 物种/宿主: HEK293 |
| 分子量: 41.9 KDa |
| 分子特征: Not available |
| 纯化: Greater than 95% as determined by reducing SDS-PAGE. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Greater than 95% as determined by reducing SDS-PAGE. | 
Figure 2. Loaded Cynomolgus IL-4RA-His on HIS1K Biosensor, can bind Human IL-4-Fc with an affinity constant of <10-3 nM as determined in BLI assay. | 
Figure 3. Loaded Human IL-4RA-His on HIS1K Biosensor, can bind Human IL-4-Fc with an affinity constant of <10-3 nM as determined in BLI assay. | 
Figure 4. Immobilized Human IL-4-Fc at 5µg/ml (100 µl/well) can bind Cynomolgus IL-4Ra-His. The ED50 of Recombinant Human IL-4Ra-His is 79.49 ng/ml. |
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