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Human ICAM-1 Protein, mFc Tag

Recombinant human ICAM-1 protein with C-terminal mouse Fc tag
5/5

¥525.00

货号:11186

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   11186
产品全名:   人 ICAM-1 蛋白
规格:   10/50/100 µg
基因符号   ICAM1;B2;D54;3.58
目标蛋白:    ICAM-1
UNIPROT ID:    P05362
描述:   Recombinant human ICAM-1 protein with C-terminal mouse Fc tag
背景:     This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
物种/宿主:    HEK293
分子量:   The protein has a predicted molecular mass of 75.7 kDa after removal of the signal peptide.The apparent molecular mass of ICAM-1-mFc is approximately 90-120 kDa due to glycosylation.
分子特征:   ICAM-1(Gln48-Glu480) mFc(Pro99-Lys330)
纯化:   The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human ICAM-1 Protein, mFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human ICAM1, mFc tagged protein (11186) can bind Anti-ICAM1 Antibody 28045 in a linear range of 0.64-80.0 ng/ml.
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