| 货号: 11253 |
| 产品全名: 人 HVEM 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 ATAR;CD270;HVEA;HVEM;LIGHTR;TR2 |
| 目标蛋白: HVEM |
| UNIPROT ID: Q92956 |
| 描述: Recombinant Human HVEM Protein with C-terminal 6xHis tag |
| 背景: This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants. |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 18.2 kDa after removal of the signal peptide.The apparent molecular mass of HVEM-His is approximately 25-55 kDa due to glycosylation. |
| 分子特征: HVEM(Leu39-Val202) 6×His tag |
| 纯化: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human HVEM Protein, His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human HVEM, His tagged protein (11253) can bind Human BTLA,mFc-His tagged protein 11174 in a linear range of 0.64-80 ng/ml. | 
Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human HVEM, His tagged protein (11253) can bind Human CD160,hFc tagged protein 11179 in a linear range of 1.28-160 ng/ml. | 
Figure 4. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human HVEM, His tagged protein (11253) can bind Human LIGHT, hFc tagged protein 11175 in a linear range of 0.32-40 ng/ml. | 
Figure 6. Flow cytometry analysis with 15 µg/ml Human HVEM Protein, His tag (11253) on Expi293 cells transfected with human LIGHT (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram). | 
Figure 6. Flow cytometry analysis with 15 µg/ml Human HVEM Protein, His tag (11253) on Expi293 cells transfected with human LIGHT (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram). |
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