| 货号: 11171 |
| 产品全名: 人 EPHA3 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 TYRO4;HEK4;ETK1;ETK;EK4;HEK |
| 目标蛋白: EPHA3 |
| UNIPROT ID: P29320 |
| 描述: Recombinant human EPHA3 protein with C-terminal mouse Fc and 6xHis tag |
| 背景: This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 85.9 kDa after removal of the signal peptide. |
| 分子特征: EPHA3(Glu21-Gln541) mFc(Pro99-Lys330) 6×His tag |
| 纯化: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human EPHA3 Protein, mFc-His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human EPHA3, mFc-His tagged protein (11171) can bind Anti-EPHA3 Neutralizing antibody 28028 in a linear range of 0.13-16.0 ng/ml. |
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