| 货号: 11833 |
| 产品全名: 人 CDH10 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 CAD10 |
| 目标蛋白: CDH10 |
| UNIPROT ID: Q9Y6N8 |
| 描述: Recombinant Human CDH10 protein with C-terminal 6xHis tag |
| 背景: This gene encodes a type II classical cadherin of the cadherin superfamily. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature cadherin protein. These integral membrane proteins mediate calcium-dependent cell-cell adhesion and are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a histidine-alanine-valine (HAV) cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is predominantly expressed in brain and is putatively involved in synaptic adhesions, axon outgrowth and guidance. Mutations in this gene may be associated with lung squamous cell carcinoma and colorectal cancer in human patients. [provided by RefSeq, Nov 2015] |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 63.0 kDa after removal of the signal peptide. The apparent molecular mass of CDH10-His is approximately 70-130 kDa due to glycosylation. |
| 分子特征: CDH10(Gly55-Ala613) 6×His tag |
| 纯化: The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human CDH10 Protein, His Tag on SDS-PAGE under reducing condition. |
