| 货号: 11142 |
| 产品全名: 人 CD28 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 CD28;Tp44 |
| 目标蛋白: CD28 |
| UNIPROT ID: P10747 |
| 描述: Recombinant human CD28 protein with C-terminal mouse Fc and 6xHis tag |
| 背景: The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 68-72 kDa after removal of the signal peptide. |
| 分子特征: CD28(Asn19-Pro152) mFc(Pro99-Lys330) 6×His |
| 纯化: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human CD28 Protein, mFc-His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human B7-1, hFc tagged protein 11283 can bind Human CD28, mFc-His tagged protein (11142) in a linear range of 125-4000 ng/ml. | 
Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human B7-2, hFc tagged protein 11284 can bind Human CD28, mFc-His tagged protein (11142) in a linear range of 62.5-4000 ng/ml. | 
Figure 4. HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28, mFc-His tagged protein (11142) 1µg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody. |
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