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Human GPR75 Full Length Protein

Human GPR75 Full Length Protein-Synthetic Nanodisc
5/5

$4,400.00

Cat.#:  11014

   Size:   50 μg

In Stock

          Product Description          
Cat.#:     11014
Product Name:   Human GPR75 Full Length Protein
规格:    10 µg, 50 µg and 100 µg
Synonyms:   GPRchr2; WI31133
Target:    GPR75
UNIPROT ID:    O95800
描述:    Human GPR75 full length protein-synthetic nanodisc
背景:     G protein-coupled receptor that is activated by the chemokine CCL5/RANTES. Probably coupled to heterotrimeric Gq proteins, it stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of this receptor.
Species/Host:    HEK293
Molecular Weight:    The human full length GPR75 protein has a MW of 59.4 kDa
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag GPR75-Nanodisc (0.2 µg/per well). Serial diluted Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for Flag monoclonal antibody binding with GPR75-Nanodisc is 3.747ng/ml.

Figure2. WB analysis of Human GPR75-Nanodisc with Flag monoclonal antibody at 1/5000 dilution, followed by Goat Rabbit IgG HRP at 1/5000 dilution
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