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Human GPR75 Full Length Protein

Human GPR75 Full Length Protein-Synthetic Nanodisc
5/5

¥30,000.00

货号:11014

规格: 50 μg

库存状态:In Stock

           产品描述          
货号:   11014
产品全名:   人 GPR75 Full Length 蛋白
规格:   10/50/100 µg
基因符号   GPRchr2; WI31133
目标蛋白:    GPR75
UNIPROT ID:    O95800
描述:   人 GPR75 full length protein-synthetic nanodisc
背景:     G protein-coupled receptor that is activated by the chemokine CCL5/RANTES. Probably coupled to heterotrimeric Gq proteins, it stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of this receptor.
物种/宿主:    HEK293
分子量:   The human full length GPR75 protein has a MW of 59.4 kDa
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag GPR75-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with GPR75-Nanodisc is 3.747ng/ml.

Figure2. WB analysis of Human GPR75-Nanodisc with anti-Flag monoclonal antibody at 1/5000 dilution, followed by Goat Anti-Rabbit IgG HRP at 1/5000 dilution
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