关于 NewEast Biosciences
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。

¥4,800.00
| Anti GαoGTP 小鼠单克隆抗体 |
| 货号: 26907 |
| 规格: 30 µL |
| 基因符号: Gnao |
| 描述: Anti Gαo-GTP 小鼠单克隆抗体 |
| 背景: Heterotrimeric G proteins are critical cellular signal transducers. Gαo represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαo proteins are being explored. |
| 免疫原: Recombinant full length protein of active Gαo |
| 经过测试的应用: IP, IHC and IF (Not applicable for WB since SDS denatures Gαo GTPase) |
| 推荐稀释度: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| 浓度: 1 mg/ml |
| 种属反应性: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG1 |
| 纯化:: Purified from ascites |
| Preservative: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: active Gαo antibody recognizes active Gαo of vertebrates. |
| 储存条件:: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation/Western blot:
Gαo Activation Assay.
Purified Gαo proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the active Gαo monoclonal antibody (Cat. No. 26907). Immunoblot was with an Gαopolyclonal antibody (Cat. No. 21015). |
| 1. The Inhibitory Role of Hydrogen Sulfide in UII-Induced Cardiovascular Effects and the Underlying Signaling Pathways Antioxidants (Basel). 2022 PMID: 36421438 |
| 2. Golgi-Resident Gao Promotes Protrusive Membrane Dynamics Cell. 2017 PMID: 28803726 |
| 3. The Gao Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal Neurons Neural Plast. 2016 PMID: 26881110 |
| 4. Wnt-5a/Frizzled9 Receptor Signaling through the Gαo-Gβγ Complex Regulates Dendritic Spine Formation J Biol Chem. 2016 PMID: 27402827 |
| 5. Toll-like receptor 2-mediated MAPKs and NF-?B activation requires the GNAO1-dependent pathway in human mast cells Integr Biol (Camb). 2016 PMID: 27515449 |
| 6. Go is required for the release of IL-8 and TNF-a, but not degranulation in human mast cells Eur J Pharmacol. 2016 PMID: 27025291 |
| 7. AKT-independent Reelin signaling requires interactions of heterotrimeric Go and Src Biochem Biophys Res Commun. 2015 PMID: 26441085 |