武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。
¥2,800.00
产品名称: Gα13 蛋白 |
货号 10138 |
产品全名: Gα13蛋白 |
基因符号 Guanine nucleotide-binding protein subunit alpha-13, Galpha 13, G13 |
Source: Human, recombinant full length, His6-tag |
Expression 种属反应性: sf9 cells |
分子量: 44 kDa |
纯化: >95% by SDS-PAGE |
Introduction: Ga13 belongs to the G12 family of heterotrimeric guanine nucleotide-binding proteins, and has intrinsic GTPase activity. Ga13 has been revealed to play critical roles in transformation, normal hemostasis and thrombosis, growth factor-induced cell migration, angiogenesis, and salt-induced hypertension. |
Amino Acid Sequence (1-377) |
MADFLPSRSVLSVCFPGCLLTSGEAEQQRKSKEIDKCLSREKTYVKRLVKILLLGAGESGKSTFLKQ MRIIHGQDFDQRAREEFRPTIYSNVIKGMRVLVDAREKLHIPWGDNSNQQHGDKMMSFDTRAPMAAQ GMVETRVFLQYLPAIRALWADSGIQNAYDRRREFQLGESVKYFLDNLDKLGEPDYIPSQQDILLARRPTK GIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVSSSEFDQVLMEDRLTNRLTESLNIFET IVNNRVFSNVSIILFLNKTDLLEEKVQIVSIKDYFLEFEGDPHCLRDVQKFLVECFRNKRRDQQQKPLYH HFTTAINTENIRLVFRDVKDTILHDNLKQLMLQ |
Properties |
Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl. |
Physical Appearance (form): White or clear |
Concentration: 1 mg/mL |
Storage: -80°C |
Preparation Instructions: Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Gα13 was determined by SDS- PAGE and Coomassie Brilliant Blue Staining. |
References:
1. Gong, H. et al., Science 327: 340-343, 2010.
2. Kabouridis, P. S. et al., Molec. Cell. Biochem. 144: 45-51, 1995. 3. Kilts, J. D. et al., J. Cardiovasc. Pharm. 50: 299-303, 2007.
4. Moers, A. et al., Nature Med. 9: 1418-1422, 2003. 5. Offermanns, S. et al, Science 275: 533-536, 1997. 6. Radhika, V. et al., J. Biol. Chem. 279: 49406-49413, 2004.
7. Ruppel, K. M. et al., Proc. Nat. Acad. Sci. 102: 8281-8286, 2005. 8. Shan, D. et al., Dev. Cell 10: 707-718, 2006.
9. Wirth, A. et al., Nature Med. 14: 64-68, 2008.
1. Receptor-interacting protein kinase 3 promotes platelet activation and thrombosis Proc Natl Acad Sci U S A. 2017 |