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MSDS

EGFR(V769L)

Anti-EGFR(V769L) Mouse Monoclonal Antibody
5/5

¥4,800.00

货号：26121

规格： 100 微升

库存状态：In Stock

           产品描述          
产品名称:   EGFR(V769L) 小鼠单抗
货号:   26121
基因符号:   EGFR/HER1/ERBB1
描述:   EGFR(V769L) 小鼠单抗
背景:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
免疫原 :   A synthetic peptide from the internal region of EGFR which includes the mutation of V769L, human origin.
Tested Applications:  ELISA, WB, If, IHC
推荐稀释比:   
ELISA: 1:1000-1:5000
WB: 1:500-1:2000
IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性：  Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes EGFR(V769L), but not wild type EGFR from vertebrates.
储存条件:   Store at -20°C. Avoid repeated freezing and thawing.

蛋白质印迹:   

Western blot analysis of recombinant EGFR(V769L) and wild type proteins.

Purified His-tagged EGFR(V769L) protein (amino acids 702-987, lane 2, 货号:10239) and corresponding wild type protein (lane 1, 货号:10210) were blotted with anti EGFR(V769L) 小鼠单抗 (货号:26121). Proteins loading (bottom) were blotted with anti-His-tag monoclonal antibody (货号:28004).

Immunofluorescence:   

Immunofluorescence of cells expressing EGFR proteins with anti EGFR(V769L) antibody.

HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP EGFR(V769L) plasmid (right column), then fixed and stained with anti- EGFR(V769L) monoclonal antibody (货号:   26121).

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           引用文献          
01. Immunohistochemistry with a novel mutation-specific monoclonal antibody as a screening tool for the EGFR L858R mutational status in primary lung adenocarcinoma
Tumor Biology. October 2014
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