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Human CGRPR-RAMP1 Full Length Protein

Human CGRPR-RAMP1 Full Length Protein-Synthetic Nanodisc
5/5

$4,400.00

Cat.#:  11126

   Size:   50 μg

In Stock

          Product Description          
Cat.#:     11126
Product Name:   Human CGRPR-RAMP1 Full Length Protein
规格:    10 µg, 50 µg and 100 µg
Synonyms:   CALCRL; CRLR; LMPHM8
Target:    CGRPR
UNIPROT ID:    Q16602
描述:    Human CGRPR-RAMP1 full length protein-synthetic nanodisc
背景:     The CGRP receptor (CGRPR) is a member of family B G protein coupled receptors (GPCRs), is expressed throughout the trigeminal system, including neurons and endothelial cells. They usually function with accessory proteins such as receptor activity modifying proteins (RAMPs) and Na/H exchange regulatory factors (NHERFs). CGRPR is a heterodimer complex of the calcitonin receptor-like receptor (CRLR) and receptor activity-modifying protein 1 (RAMP1). Therapeutics for migraine treatment are mostly targeting CRLR-RAMP1 protein-protein interaction surfaces, thereby blocking CGRP activity.
Species/Host:    HEK293
Molecular Weight:    The human full length CGRPR protein has a MW of 53.0 kDa
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag CGRPR-RAMP1-Nanodisc (0.2 µg/per well). Serial diluted CGRPR monoclonal antibody (28157) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for CGRPR monoclonal antibody binding with CGRPR-RAMP1-Nanodisc is 1216ng/ml.

Figure2. Human CGRPR-RAMP1-Nanodisc, Flag Tag on SDS-PAGE
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