武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。
¥4,800.00
产品名称: BRAF(G469E) 小鼠单抗 |
货号: 26196 |
基因符号: BRAF, BRAF1, RAFB1 |
描述: BRAF(G469E) 小鼠单抗 |
背景: BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. |
免疫原: A synthetic peptide from the internal region of BRAF which includes the mutation of G469E, human origin. |
经过测试的应用: ELISA, WB, IHC |
推荐稀释比: ELISA: 1:1000-1:5000WB: 1:500-1:1000IHC: 1:50-1:100 |
Concentration: 1mg/ml |
种属反应性: Mouse |
形式: Liquid |
克隆性: Monoclonal |
亚型: IgG |
纯化: Purified from ascites |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Recognizes G469E mutant, but not wild type BRAF of vertebrates. |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: Western blot analysis of recombinant BRAF (G469E) and wild type proteins.Purified His-tagged BRAF (G469E) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-BRAF(G469E) monoclonal antibody (货号: 26196).Immunofluorescence: Immunofluorescence of cells expressing BRAF proteins with Anti-BRAF(G469E) antibody.HEK293T cells were transfected with pCDNA3-GFP-BRAF (WT) plasmid (left column) or pCDNA3-GFP-BRAF (G469E) plasmid (right column), then fixed and stained with Anti-BRAF(G469E) monoclonal antibody (货号: 26196). |
1. Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma Mod Pathol. 2013 Mar;26(3):414-20. |
2. Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733 |
3. Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma human pathology. February 2015Volume 46, Issue 2, Pages 169–175 |
4. Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570 |
5. BRAF V600E Mutation-Specific Antibody: A Review Seminars in Diagnostic Pathology |