武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。
¥2,600.00
产品名称: BRaf 兔多抗 |
货号: 21271 |
基因符号: B-RAF, B-RAF1, RAFB1 |
描述: BRaf 兔多抗 |
背景: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the B-RAF gene cause diseases. Inherited mutations in B-RAF cause cardiofaciocutaneous syndrome. Acquired mutations in B-RAF have been found in cancers. |
免疫原 : Purified B-Raf protein, human origin. |
经过测试的应用: ELISA, WB, IHC |
推荐稀释比: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:200 |
Concentration: 0.1mg/ml |
种属反应性: Rabbit |
形式: Liquid |
克隆性: Polyclonal |
亚型: |
纯化: Purified from serum |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Recognizes B-Raf of vertebrates. |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry:
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti- B-Raf 兔多抗 (货号:21271). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.
1. Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma Mod Pathol. 2013 Mar;26(3):414-20. |
2. Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733 |
3. Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma human pathology. February 2015Volume 46, Issue 2, Pages 169–175 |
4. Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570 |
5. BRAF V600E Mutation-Specific Antibody: A Review Seminars in Diagnostic Pathology |