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MSDS

BRaf pAb

Anti-BRaf Rabbit Polyclonal Antibody
5/5

¥2,600.00

货号：21271

规格： 100 微升

库存状态：In Stock

           产品描述          
产品名称:   BRaf 兔多抗
货号:   21271
基因符号:   B-RAF, B-RAF1, RAFB1
描述:   BRaf 兔多抗
背景:   B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the B-RAF gene cause diseases. Inherited mutations in B-RAF cause cardiofaciocutaneous syndrome. Acquired mutations in B-RAF have been found in cancers.
免疫原 :   Purified B-Raf protein, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:200
Concentration:    0.1mg/ml
种属反应性:   Rabbit
形式:   Liquid
克隆性：  Polyclonal
亚型:   
纯化:   Purified from serum
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes B-Raf of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry:   

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti- B-Raf 兔多抗 (货号:21271). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.

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           引用文献          
1.  Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
Mod Pathol. 2013 Mar;26(3):414-20.
2.  Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733
3.  Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
human pathology. February 2015Volume 46, Issue 2, Pages 169–175
4.  Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570
5.  BRAF V600E Mutation-Specific Antibody: A Review
Seminars in Diagnostic Pathology
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