蛋白质印迹:   

Western blot analysis of recombinant ATM protein. Purified His-tagged ATM protein was blotted with Anti-ATM 兔多抗 (货号:21276).

Immunohistochemistry:   

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Anti-ATM 兔多抗 (货号:21276). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.