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Arf6 Protein

Arf6 Protein
5/5

¥2,800.00

货号:10124

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   Arf6 蛋白
货号:   10124
产品全名:   Arf6 蛋白
基因符号  ADP-ribosylation factor 6
Source:   人 recombinant, His6-tag
Expression 种属反应性:   E. coli
分子量:   17 kDa
纯化:   >95% by SDS-PAGE
Introduction:   Arf6 is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf6 regulates membrane trafficking and the actin cytoskeketon at the plasma membrane and functions as a regulatory molecule of phagocytosis.
Amino Acid Sequence  (13-175)
EMRILMLGLDAAGKTTILYKLKLGQSVTTIPTVGFNVETVTYKNVKFNVWDVGGQDKIRPLWRHYYT
GTQGLIFVVDCADRDRIDEARQELHRIINDREMRDAIILIFANKQDLPDAMKPHEIQEKLGLTRIRD
RNWYVQPSCATSGDGLYEGLTWLTSNYKS
Properties
Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form):   White or clear
Concentration:   1 mg/mL
Storage:    -80°C
Preparation Instructions:   
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Arf6 was determined by SDS- PAGE and Coomassie Brilliant Blue Staining.


References:   
1. Cavenagh, M. M. et al., J. Biol. Chem. 271: 21767-21774, 1996.
2. D’Souza-Schorey, C. et al., Science 267: 1175-1178, 1995.
3. Falace, A. et al., Am. J. Hum. Genet. 87: 365-370, 2010.
4. Hernandez-Deviez, D. J. et al., Nature Neurosci. 5: 623-624, 2002.
5. O’Neal, C. J. et al., Science 309: 1093-1096, 2005.

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