| 货号: S218254 |
| 产品全名: ZNF496 兔多抗 |
| 基因符号 NIZP1; ZFP496; ZSCAN49; ZKSCAN17 |
| UNIPROT ID: Q96IT1 (Gene Accession – BC007263 ) |
| 背景: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZNF496 (Zinc finger protein 496), also known as ZKSCAN17 or NIZP1, is a 587 amino acid member of the Krppel C2H2-type zinc-finger protein family and is thought to act as a transcriptional repressor. Localized to the nucleus, ZNF496 contains one SCAN box domain, one KRAB domain and five C2H2-type zinc fingers through which it may convey DNA, RNA and protein binding capabilities. |
| 抗原: Fusion protein of human ZNF496 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 200-1000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 218254(ZNF496 Antibody) at a dilution of 1/30(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 218254(Anti-ZNF496 Antibody) at dilution 1/30. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: RAW264.7, SP20, A431, HEPG2, NIH/3T3 and 293T cell lysates;
Primary antibody: 218254(ZNF496 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds | |
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