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ZAP70 ? Rabbit pAb

Anti-ZAP70 ? Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S213311

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S213311
产品全名:   ZAP70 兔多抗
基因符号   SRK; STD; TZK; STCD; ZAP-70
UNIPROT ID:   P43403 (Gene Accession – NP_001070 )
背景:   This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
抗原:   Synthetic peptide of human ZAP70
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Immunology
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 213311(ZAP70 Antibody) at a dilution of 1/50(Cytoplasm ).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 213311(Anti-ZAP70 Antibody) at dilution 1/50.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane: Jurkat cells;
Primary antibody: 213311(ZAP70 Antibody) at dilution 1/1000;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
           引用文献