| 货号: S219935 |
| 产品全名: YWHAB 兔多抗 |
| 基因符号 HS1, GW128, YWHAA, KCIP-1 |
| UNIPROT ID: P31946 (Gene Accession – NP_003395 ) |
| 背景: This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. |
| 抗原: Synthetic peptide of human YWHAB |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 1000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Neuroscience |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 219935(YWHAB Antibody) at a dilution of 1/30(Cytoplasm, Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the synthetic peptide and then with 219935(Anti-YWHAB Antibody) at dilution 1/30. | 
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 219935(Anti-YWHAB Antibody) at a dilution of 1/30. | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with synthetic peptide and then with D260652(Anti-YWHAB Antibody) at dilution 1/30. | 
Gel: 12%SDS-PAGE, Lysate: 30 μg;
Lane 1-2: Hela cells, mouse kidney tissue;
Primary antibody: 219935(YWHAB Antibody) at dilution 1/550;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds | |
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