| 货号: S218284 |
| 产品全名: WDFY2 兔多抗 |
| 基因符号 PROF; WDF2; ZFYVE22 |
| UNIPROT ID: Q96P53 (Gene Accession – BC014004 ) |
| 背景: WD repeat and FYVE domain-containing protein 2 (WDFY2), also known as WDF2 and ZFYVE22, is a 400 amino acid protein that localizes to a set of small endosomes that are found within 100 nm from the plasma membrane. Highly conserved between species, WDFY2 consists of one FYVE-type zinc finger and seven WD repeats. The FYVE domain is a cysteine-rich domain of about 70 amino acids. Its primary role is to target signal-transducing proteins to cell membranes through binding to the membrane lipid phosphatidylinositol-3-phosphate with high specificity. WD-repeats are generally found in clusters of seven. They have no intrinsic catalytic activity, but they serve as a platform for protein-protein interactions. WDFY2 is suspected to play a critical role in the endocytic pathway. |
| 抗原: Fusion protein of human WDFY2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 30-150;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 218284(WDFY2 Antibody) at a dilution of 1/40(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 218284(Anti-WDFY2 Antibody) at dilution 1/40. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse kidney tissue lysate;
Primary antibody: 218284(WDFY2 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 15 seconds | |
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