| 货号: S217205 |
| 产品全名: VWA5A 兔多抗 |
| 基因符号 BCSC1; BCSC-1; LOH11CR2A |
| UNIPROT ID: O00534 (Gene Accession – BC001234 ) |
| 背景: VWA5A (von Willebrand factor A domain containing 5A), also known as BCSC-1 (breast cancer suppressor candidate 1) or LOH11CR2A (loss of heterozygosity 11 chromosomal region 2 gene A protein), is a 786 amino acid protein containing one VIT domain and one VWFA domain. VWA5A is expressed at low levels in various tissues, with no expression found in 80% of tumor cell lines. Likely acting as a tumor suppressor gene, deletion of VWA5A leads to loss of heterozygosity (LOH) in breast and ovarian tumors, and may have an important role as a potential gene therapy target. Abnormal expression of VWA5A may lead to an increase in adhesion of CNE-2L2 cells associated with an increase in expression of E-cadherin, alpha-catenin, and p53, resulting in a decrease of malignant activity in cells with ectopic expression of VWA5A. |
| 抗原: Fusion protein of human VWA5A |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 100-300;WB: 500-2000;ELISA: 2000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 217205(VWA5A Antibody) at a dilution of 1/60(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 217205(Anti-VWA5A Antibody) at dilution 1/60. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 217205(Anti-VWA5A Antibody) at a dilution of 1/60. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D221980(Anti-VWA5A Antibody) at dilution 1/60. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Human Adrenal pheochromocytoma tissue, Jurkat cells, A549 cells;
Primary antibody: 217205(VWA5A Antibody) at dilution 1/275;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds | |
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