| 货号: N263079 |
| 产品全名: VPS4A 兔单克隆抗体 |
| 基因符号 vacuolar protein sorting 4 homolog A; SKD1; SKD2; VPS4; SKD1A; VPS4-1 |
| UNIPROT ID: Q9UN37 |
| 背景: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (PubMed:24814515). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). |
| 抗原: A synthetic peptide of human VPS4a |
| 经过测试的应用: WB,IHC-F,IHC-P,ICC/IF |
| 推荐稀释比: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Monoclonal |
| 克隆编号: R01-9H9 |
| 分子量: Calculated MW: 49 kDa; Observed MW: 49 kDa |
| 亚型: IgG |
| 纯化: Affinity Purified |
| 种属反应性: Human, Mouse and Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunocytochemistry analysis of VPS4a(green) in Hela using VPS4a antibody,and DAPI(blue) | 
Western blot analysis of VPS4a in K562, rat Brain, C6, 3T3 lysates using VPS4A antibody. | 
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using VPS4a antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. | |
|
