| 货号: S218326 |
| 产品全名: USP12 兔多抗 |
| 基因符号 UBH1; USP12L1 |
| UNIPROT ID: O75317 (Gene Accession – BC026072 ) |
| 背景: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP12 (ubiquitin specific peptidase 12), also known as UBH1 or USP12L1, is a 370 amino acid protein belonging to the peptidase C19 family and the USP12/USP46 subfamily. Considered a deubiquitinating enzyme, it is suggested that USP12 has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have high activity. The gene encoding USP12 maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome. |
| 抗原: Full length fusion 蛋白 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218326(USP12 Antibody) at a dilution of 1/35(Cytoplasm and Cell membrane). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218326(Anti-USP12 Antibody) at dilution 1/35. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse liver tissue lysate;
Primary antibody: 218326(USP12 Antibody) at dilution 1/700;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 second | |
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