| 货号: S215332 |
| 产品全名: UBE2N 兔多抗 |
| 基因符号 UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13 |
| UNIPROT ID: P61088 (Gene Accession – NP_003339 ) |
| 背景: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. |
| 抗原: Synthetic peptide of human UBE2N |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Cell Biology, Immunology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 215332(UBE2N Antibody) at a dilution of 1/55(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 215332(Anti-UBE2N Antibody) at dilution 1/55. | 
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 215332(Anti-UBE2N Antibody) at a dilution of 1/55. | 
In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D163219(Anti-UBE2N Antibody) at dilution 1/55. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Mouse brain tissue, Hela cell, LNCAP cell, HepG2 cell, Jurkat cell lysates;
Primary antibody: 215332(UBE2N Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds | |
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