| 货号: S218112 |
| 产品全名: UBE2J2 兔多抗 |
| 基因符号 UBC6; NCUBE2; NCUBE-2; PRO2121 |
| UNIPROT ID: Q8N2K1 (Gene Accession – BC113430 ) |
| 背景: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. |
| 抗原: Fusion protein of human UBE2J2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218112(UBE2J2 Antibody) at a dilution of 1/80(Cytoplasm and Cell membrane). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218112(Anti-UBE2J2 Antibody) at dilution 1/80. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 218112(Anti-UBE2J2 Antibody) at a dilution of 1/80. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D223744(Anti-UBE2J2 Antibody) at dilution 1/80. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HepG2, 293T cell lysates;
Primary antibody: 218112(UBE2J2 Antibody) at dilution 1/1200;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 6 seconds | |
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