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UBE2I Rabbit pAb

Anti-UBE2I Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S221208

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S221208
产品全名:   UBE2I 兔多抗
基因符号   P18; UBC9; C358B7.1
UNIPROT ID:   P63279 (Gene Accession – NP_003336 )
背景:   The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
抗原:   Synthetic peptide of human UBE2I
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Epigenetics and Nuclear Signaling, Metabolism, Cell Biology
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221208(UBE2I Antibody) at a dilution of 1/40(Nucleus and Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221208(Anti-UBE2I Antibody) at dilution 1/40.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: TM4, hela, 293T and Jurkat cell;
Primary antibody: 221208(UBE2I Antibody) at dilution 1/650;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
           引用文献